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Dr. Zygmunt Gryczynski

GryczynskiZygmunt
"Tex" Moncrief Jr. Chair Professor of Physics Main: (817) 257-4209

Biography

Dr. Zygmunt Gryczynski received his M.S. in experimental physics in 1982 from the University of Gdansk and Ph.D. in spectroscopy in 1987, working on the basic spectroscopic studies of isotropic and oriented systems of organic molecules. In 1991 he becomes a Research Assistant Professor in the Department of Biochemistry and Molecular Biology, University of Maryland and from 1998 to 2005 he was an Assistant Director in the Center for Fluorescence Spectroscopy at the University of Maryland. From 2005 he is a Professor of Molecular Biology and Immunology at the University of North Texas Health Science Center at Fort Worth, Texas. In 2006 with the support from the Emerging Technology Funds (ETF) of Texas together with his colleagues he established a Center for Commercialization of Fluorescence Technologies (CCFT) that in 2013 has been transformed to the Center for Fluorescence Technologies and Nanomedicine (CFTN). In 2010 he becomes the “Tex” Moncrief Jr. Chair and Professor of Physic in the Department of Physics and Astronomy, Texas Christian University at Fort Worth. His present appointments also include Visiting Professor, Department of Physics, University of Strathclyde, Glasgow, UK and Adjunct Professor, University of Shimane, Matsue, Japan. His early work at the University of Maryland was focused on ultrafast time-resolved fluorescence spectroscopy and intrinsic fluorescence of hemoproteins as well as the thermodynamics of ligand binding and the allosteric mechanism of O2 binding in hemoproteins. He has been also developing uses of multi-photon excitation and light quenching (light stimulated emission) in time-resolved fluorescence spectroscopy. His focus has been on applications of fluorescence spectroscopy to study biological systems using time-resolved fluorescence, anisotropy, and FRET. He also pioneered novel fluorescence sensing methods for biomedical applications in tissue and blood. More recently his interests expanded to nanotechnology and applications of novel plasmonic effects induced by light and excited fluorophore in metallic nanostructures. He pioneered metal enhanced fluorescence (MEF) and surface plasmons coupled emission (SPCE) phenomena for biomedical and diagnostics application (biophotonics). His current focus is to develop novel fluorescent probes and to explore quantum-level interactions to study the dynamics of biophysical and biochemical processes at the molecular level and development of new fluorescence probes for studying molecular interactions and dynamics on the cellular level. He worked and published with two Nobel Prize laureates: S. Prusiner (1997) and B. Kobilka (2012).  Authored over 300 peer-review publications, 12 book chapters, 8 patents, and edited 13 books. He is also a member of Editorial Boards of Journal of Experimental Biology and Medicine and Methods and Applications in Fluorescence.

Research Interests

  • Spectroscopy and Fluorescence: Absorption; Linear Dichroism; Fluorescence; Fluorescence Polarization; Phosphorescence
  • Probe Development. Novel fluorophores for molecular and cellular imagingOrganic fluorophores; Quantum dots; Nanoparticles
  • Time-Resolved Spectroscopy: Time-resolved fluorescence and phosphorescence; Excited state processes/dynamics.
  • Forster Resonance Energy Transfer (FRET): Steady-state and time-resolved applications at molecular and cellular levels.
  • Molecular/Macro-Molecular Dynamics: Protein Dynamics; Protein Dissociation/Associatio; DNA/RNA.
  • Cooperative Binding: Oxygen binding/transport by hemoglobin; Ligand binding; Macromolecular binding.
  • Biomarkers – Enhanced Fluorescence Based Detection. Cardiac Risk Assessment and Cancer Detection.
  • Imaging: Tissue; Cell; Single molecule.
  • Fluorescence Based Sensing: Lifetime based sensing; Novel sensing; Plasmons enhanced sensing.
  • Multi-Photon Processes: Multi-Photon Induced Fluorecence (Two-Photon, Three-Photon, Four-Photon Excitation); Light Quenching by Stimulated Emission; Two-Pulse Fluorescence.
  • Total Internal Reflection Fluorescence: Steady-state; Time-resolved.
  • Metal Enhanced Fluorescence (MEF): Metallic nanoparticles/nanostructures enhanced detection; Localized surface plasmons.
  • Surface Plasmons Enhanced Detection (SPED): Surface plasmon enhanced fluorescence (SPCE); Surface plasmon resonance (SPR) stimulated emission.
  • Optical and Quantum Processes to Study Biological System in Nanoscale: Proteins, DNA/RNA, Cells, Tissue

Publications

Peer-reviewed only ~300 total; last 3 years only

  • Differences in the spatial distribution of actin in the left and right ventricles of functioning rabbit hearts. J Nagwekar, D Duggal, R Rich, R Fudala, I Gryczynski, S Raut, Z Gryczynski, J BorejdoMedical Photonics, 2016, in press.
  • Spectroscopic differentiation between monomeric and aggregated forms of BODIPY dyes: effect of 1, 1-dichloroethane. M. Castillo, S. L. Raut,b S. Price, Ilkay Bora, L. P. Jameson, C. Qiu, K. A. Schug, Z. Gryczynski and S. V. Dzyuba. RCA Adv. 2016, 6, 68705-8.
  • Linear dichroism and optical anisotropy of silver nanoprisms in polymer films. S. Requena, H. Doan, S. Raut, A. D’Achille, Z. Gryczynski, I. Gryczynski, Y. M. Strzhemechny. Nanotechnology, 2016, 27, 325704 (6pp). doi:10.1088/0957-4484/27/32/325704.
  • Mechanothermally induced conformational switch of a porphyrin dimer in a polymer film. H. Doan, S. L. Raut, D. Yale, M. Balaz, S. V. Dzyuba and Z. Gryczynski. Chem. Commun., 2016, 52, 9510-9513.
  • Two blinking mechanisms in highly confined AgInS2 and AgInS2/ZnS quantum dots evaluated by single particle spectroscopy.Cichy B, Rich R, Olejniczak A, Gryczynski Z, Strek W.  Nanoscale. 2016 Feb 11;8(7):4151-9. doi: 10.1039/c5nr07992f. PubMed PMID: 26866468.
  • Defect-mediated spontaneous emission enhancement of plasmon-coupled CuInS2 and CuInS2/ZnS. Rice Q, Raut S, Chib R, Hayes A, Gryczynski, Z., Gryczynski I, Kim Y, Tabibi B, and Seo.J.Optical Matrial Express. 2016, 6 (2), 566-577.
  • Fluorescent biosensor for the detection of hyaluronidase: intensity-based ratiometric sensing and fluorescence lifetime-based sensing using a long lifetime azadioxatriangulenium (ADOTA) fluorophore. Chib R, Mummert M, Bora I, Laursen BW, Shah S, Pendry R, Gryczynski I, Borejdo J, Gryczynski Z, Fudala R.. Anal Bioanal Chem. 2016 Mar 18. PubMed PMID: 26993308.
  • Triazine-based BODIPY trimer as a molecular viscometer. Raut, S., J. Kimball, R. Fudala, I. Bora, R. Chib, H. Jaafari, M. Castillo, N. Smith, S. Dzyuba, Z. Gryczynski. Physical Chemistry Chemical Physics. 2016. DOI:10.1039/C5CP07214J
  • Photophysical characterization of anticancer drug valrubicin in rHDL nanoparticles and its use as an imaging agent. Shah, S., R Chib, S. Raut, J. Bermudez, N. Sabnis, D. Duggal, J.D. Kimball, A. Lacko, Z. Gryczynski, I. Gryczynski. J. Photochem. Photobiol. B: Biology 2016, 155, 60-65.
  • Effect of quencher, denaturants, temperature and pH on the fluorescent properties of BSA protected gold nanoclusters. R Chib, S Butler, S Raut, S Shah, J Borejdo, Z Gryczynski, I Gryczynski, J. of Luminescence, 2015, 168, 62-68.
  • MIEN1, a novel interactor of Annexin A2, promotes tumor cell migration by enhancing AnxA2 cell surface expression, Kpetemey, M., S Dasgupta, S Rajendiran, S Das, LD Gibbs, P Shetty, Z. Gryczynski, J. K Vishwanatha, 2015, Molecular Cancer 14 (1), 1
  • A Novel Method of Determining the Functional Effects of a Minor Genetic Modification of a Protein. Nagwekar, J., D Duggal, K Midde, R Rich, J Liang, K Kazmierczak, W. Huang, R. Fudala, I. Gryczynski, Z. Gryczynski, D. Szczesna-Cordary, J. Borejdo, Frontiers in Cardiovascular Medicine 2015, 2
  • Standard reference for instrument response function in fluorescence lifetime measurements in visible and near infrared. Chib, R., S Shah, Z Gryczynski, R Fudala, J Borejdo, B Zelent, M. G Corradini, R. D Ludescher, I. Gryczynski. Measurement Science and Technology 2015, 27 (2), 027001
  • Effect of quencher, denaturants, temperature and pH on the fluorescent properties of BSA protected gold nanoclusters.Chib, R., S Butler, S Raut, S Shah, J Borejdo, Z Gryczynski, I Gryczynski, J. of Luminescence, 2015, 168, 62-68.
  • Sandwich type plasmonic platform for MEF using silver fractals. S. L Raut, R. Rich, T. Shtoyko, I. Bora, B. W Laursen, T. J. Sørensen, J. Borejdo, Z. Gryczynski, I. Gryczynski. Nanoscale, 2015, 7, 17729-17734
  • Methylene Blue Protects Astrocytes against Glucose Oxygen Deprivation by Improving Cellular Respiration. R. Choudhury G, Winters A, Rich RM, Ryou M-G, Gryczynski Z, Yuan, F., Yang S-H, Liu, R. (2015) PLoS ONE 10(4): e0123096. doi:10.1371/journal.pone.0123096
  • Physicochemical properties of potential porphyrin photosensitizers for photodynamic therapy. M. Kempa, P. Kozub, J. Kimball, M. Rojkiewicz, P. Kuś, Z. Gryczyński, A. Ratuszna. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 146 (2015) 249–254.
  • Steady state and time resolved fluorescence studies of azadioxatriangulenium (ADOTA) fluorophore in silica and PVA thin films. (2015) R Chib, S Raut, S Shah, B Grobelna, I Akopova, R Rich, TJ Sørensen, Z. Gryczynski, I. Gryczynski Dyes and Pigments 117, 16-23
  • BODIPY–BODIPY dyad: assessing the potential as a viscometer for molecular and ionic liquids (2015) JD Kimball, S Raut, LP Jameson, NW Smith, Z Gryczynski, SV Dzyuba, RSC Advances 5 (25), 19508-19511
  • The Spatial Distribution of Actin and Mechanical Cycle of Myosin Are Different in Right and Left Ventricles of Healthy Mouse Hearts. (2014) J Nagwekar, D Duggal, R Rich, S Raut, R Fudala, I Gryczynski, Z. Gryczynski, J. Borejdo, Biochemistry 53 (48), 7641-7649
  • Preparation of Plasmonic Platforms of Silver Wires on Gold Mirrors and Their Application to Surface Enhanced Fluorescence. Shtoyko, T., S. Raut, R. Rich, R. J Sronce, R. Fudala, R. N Mason, I. Akopova, Z. Gryczynski, I. Gryczynski. 2014, Applied Materials and Interfaces. Vol. 6, 18780−18787
  • Fractional Contributions of Defect-Originated Photoluminescence from CuInS 2/ZnS Coreshells for Hybrid White LEDs. Rice, Q., S. Raut, R. Chib, Z. Gryczynski, I. Gryczynski, W. Zhang, X. Zhong, M. Abdel-Fattah, B. Tabibi, J. Seo. 2014, Journal of Nanomaterials, Vol 2014, Article ID 979875, 7 pages.
  • A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environments. Raut, S., J Kimball*, R Fudala, H Doan*, B Maliwal, N Sabnis, A Lacko, I. Gryczynski, S. V. Dzyuba, Z. Gryczynski, 2014, Physical Chemistry Chemical Physics 16 (48), 27037-27042
  • Generating multiple-pulse bursts for enhanced fluorescence detection. (2014) Shumilov D., Rich, R.M., Gryczynski, I., Raut, S., Gryczynski, K., Kimball, J., Doan, H., Sorensen, T. J., Laursen, B., W., Borejdo, J., Gryczynski, Z.Methods Appl Fluoresc. 2, 024009 (9pp).
  • Associated anisotropy decays of ethidium bromide interacting with DNA. R. Chib, S. Raut, Sarika Sabnis, Preeti Singhal, Z. Gryczynskiand I. Gryczynski. Methods Appl. Fluoresc. 2 (2014)
  • Evidence of energy transfer from tryptophan to BSA/HAS protected gold nanoclusters. (2014) Raut, S., Chib, R., Butler, S., Borejdo, J., Gryczynski, Z., Gryczynski, I. Methods Appl. Fluoresc. 2, 035004 (6pp).
  • Resonance energy transfer between fluorescent BSA protected Au nanoclusters and organic fluorophores. Sangram Raut, Ryan Rich, Rafal Fudala, Susan Butler, Rutika Kokate, Z. Gryczynski, Rafal Luchowski, Ignacy Gryczynski. Nanoscale, 2014,6, 385-391.
  • Long lived BSA Au clusters as a time gated intensity imaging probe. SL Raut, R Fudala, R Rich, RA Kokate, R Chib, Z Gryczynski, I Gryczynski. Nanoscale, 2014,6, 2594-2597
  • Generating multiple-pulse bursts for enhanced fluorescence detection. Dmytro Shumilov, Ryan M Rich, Ignacy Gryczynski, Sangram Raut, Karol Gryczynski, Joe Kimball, Hung Doan, Thomas J Sørensen, Bo W Laursen, Julian Borejdo, Zygmunt Gryczynski. Methods Appl. Fluoresc. 2 (2014) 024009, doi:10.1088/2050-6120/2/2/024009
  • Multiple-pulse pumping for enhanced fluorescence detection and molecular imaging in tissue. Rich RM, Gryczynski I, Fudala R, Borejdo J, Stankowska DL, Krishnamoorthy RR, Raut S, Maliwal BP, Shumilov D, Doan H, Gryczynski Z. (2014) Methods 66, 292-298.
  • Real-time imaging of exocytotic mucin release and swelling in Calu-3 cells using Acridine Orange. Shumilov D, Popov A, Fudala R, Akopova I, Gryczynski I, Borejdo J, Gryczynski Z, Grygorczyk R. (2014) Methods 66, 312-324.
  • Multiple-Pulse Pumping with Time-Gated Detection for Enhanced Fluorescence Imaging in Cells and Tissue. Fudala, R., R. M. Rich, J. Kimball*, I. Gryczynski, S. Raut, J. Borejdo, D. L Stankowska, R. R Krishnamoorthy, K. Gryczynski, B. P Maliwal, Gryczynski, Z., 2014 Springer Series of Fluorescence. Pp. 1-15. Springer Berlin Heidelberg, 2014.
  • Effect of Ionic Liquids on the Conformation of a Porphyrin-Based Viscometer. L.P. Jameson, J. D. Kimball, Z. Gryczynski, M. Balaz, S. V. Dzyuba. (2013) RSC Adv. 3, 18300-18304.
  • Resonance Energy Transfer Between Fluorescent BSA Protected Au Nanoclusters and Organic Fluorophores.Raut S, Rich R, Fudala R, Butler S, Kokate R, Gryczynski Z, Luchowski R, Gryczynski I. (2013) Nanoscale 6, 385-391
  • Seo J-T, Raut S, Abdel-Fattah M, Rice Q, Tabibi B, Rich R, Fudala R, Gryczynski I, Gryczynski Z, Kim W-J, Jung S, Hyun R. Time-resolved and temperature-dependent photoluminescence of ternary and quaternary nanocrystals of CuInS2 with ZnS capping and cation exchange. J. Appl. Phys. 114, 094310 (2013); doi: 10.1063/1.4820269
  • Long-lived bright red emitting azaoxo-triangulenium fluorophores.Maliwal BP, Fudala R, Raut S, Kokate R, Sørensen TJ, Laursen BW, Gryczynski Z, Gryczynski I. PLoS One. (2013) May 7;8(5):e63043.
  • Elimination of autofluorescence in fluorescence correlation spectroscopy using the AzaDiOxaTriAnulenium (ADOTA) fluorophore in combination with time-correlated single-photon counting (TCSPC). (2013) Rich, R.M., Mummert, M., Gryczynski, Z., Borejdo, J., Sorensen, T.J., Laursen, B.W., Foldes-Papp, Z., Gryczynski, I., Fudala, R. Anal. Bioanal. Chem. 405, 4887-4894.
  • Bruton’s Tyrosine Kinase Mediates FcgammaRIIa/Toll-like Receptor-4 Receptor Crosstalk in Human Neutrophils. (2013) Krupa, A., Fudala, R., Florence, J.M., Tucker, T., Allen, T.C., Standiford, T.J., Luchowski, R., Fol, M., Rahman, M., Gryczynski, Z., Gryczynski, I., Kurdowska, A.K. Am. J. Respir. Cell. Mol. Biol. 48, (2), 240-249.
  • AzadioxaTriAnulenium: a long fluorescence lifetime fluorophore for large biomolecule binding assay. (2013) Sorensen, T.J., Thyrhaug, E., Szabelski, M., Gryczynski, I., Gryczynski, Z., Laursen, B.W. Methods Appl. Fluoresc. 1, 025001(6pp).
  • FRET Based Ratio-Metric Sensing of Hyaluronidase in Synthetic Urine as a Biomarker for Bladder and Prostate Cancer. (2013) Chib, R., Raut, S., Fudala, R., Chang, A., Mummert, M., Rich, R., Gryczynski, Z., Gryczynski, I. Curr. Pharmac. Biotechn. 14, 470-474.
  • Elimination of autofluorescence background from fluorescence tissue images by use of time-gated detection and the AzaDiOxaTriAngulenium (ADOTA) fluorophore. (2013) Rich, R.M., Stankowska, D.L., Maliwal, B.P., Sorensen, T.J., Laursen, B.W., Krishnamoorthy, R.R., Gryczynski, Z., Borejdo, J., Gryczynski, I., Fudala, R.. Anal. Bioanal. Chem. 405, 2065-2075.
  • Two photon induced luminescence of BSA protected gold clusters. (2013) Raut, S. L., Shumilov, D., Chib, R., Rich, R., Gryczynski, Z., Gryczynski, I.. Chem. Phys. Lett. 561-562, 74-76.
  • Polarization properties of fluorescent BSA protected Au25 nanoclusters. (2013) Raut, S., Chib, R., Shumilov, D., Gryczynski, Z., Gryczynski, I.. Nanoscale 5, 3441-3446.

Patents

Polarization Based Sensing, US Patent # 6395556

Devices and Methods for Monitoring Drug Therapy Compliance. US Patent #6663846

Nanoparticles as Luminescent Probes. US Patent # 6,660,379

Apparatus and Methods for Surface-Plasmon Coupled Directional Emission. US Patent # 20050053974A1

Optical Structures for Metal-Enhanced Sensing. US Patent # 7,095,502

Surface Plasmon Assisted Microscopy. US Patent # 7956989 B2

Ratiometric Surface Plasmon Coupled Emission Detector. US Patent # 8159676 B2

Polarization standards for microscopy. US Patent # US 20110089317 A1

FRET Based Multiplex Probes. US Patent # US 20140274797 A1